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i want to calculate neb from nebmake.pl script file. so it is showing error that in vasp.pm:: read_poscar, cannot open POSCAR00

I assume that you are referring to the nebmake.pl script provided here:
https://theory.cm.utexas.edu/vtsttools/scripts.html

We (the VAPS team) don't provide support for these scripts.
You might try to ask your question on the Bug Tracker:
http://theory.cm.utexas.edu/redmine/pro ... ols/issues

Perhaps some other user of the forum with experience on this script might be able to help you.

can u tell me??? how can u calculate neb with out perl script

Yes, we have a NEB tutorial here:
https://www.vasp.at/wiki/index.php/Coll ... alculation

And documentation here:
https://www.vasp.at/wiki/wiki/index.php/IMAGES

i want to freeze all atoms except the atom which is migrating one place to another...then i am getting very high energy barrier compared to without freeze